Journal: Gastric Cancer

Article Title: Sustained exposure to Helicobacter pylori induces immune tolerance by desensitizing TLR6

doi: 10.1007/s10120-023-01461-7

Figure Lengend Snippet: Rac1, pPI3K, tPI3K, pGSK-3β, tGSK-3β, NF-κB p50 and NF-κB p65, pJNK, tJNK, pJUN and tJUN expression in control cells, Hp- 1 cells and Hp- 30 cells ( A ). TLR6 and pJNK expression in control cells, Hp- 1 cells, GES-1 cells treated with 1 μM C29 for 24 h and GES-1 cells treated with H. pylori and 1 μM C29 for 24 h ( B ). TLR6 and pJNK expression in control cells, GES-1 cells treated with 0.25 μg/mL, 0. 5 μg/mL, 1 μg/mL and 2 μg/mL Pam2CSK4 for 24 h ( C ). TLR6 and pJNK expression in GES-1 cells, Hp- 1 cells, GES-1 cells treated with 1 μg/mL Pam2CSK4 for 24 h, GES-1 cells treated with 2 μg/mL SP600125 for 24 h, GES-1 cells treated with H. pylori and 2 μg/mL SP600125 for 24 h and GES-1 cells treated with 1 μg/mL Pam2CSK4 and 2 μg/mL SP600125 for 24 h ( D ). All experiment were performed in triplicate and all data represents means ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The TLR2/6 heterodimer agonist Pam2CSK4 was purchased from Apexbio (B5665).

Techniques: Expressing, Control

Journal: Gastric Cancer

Article Title: Sustained exposure to Helicobacter pylori induces immune tolerance by desensitizing TLR6

doi: 10.1007/s10120-023-01461-7

Figure Lengend Snippet: TLR6 mRNA expression ( A ) and IL-1β mRNA expression ( B ) in Hp- 30 cells, Hp- 30 cells co-culture with H. pylori for 24 h and Hp -30 cells co-culture with H. pylori and 10 μg/ml Pam2CSK4 for 24 h. Supernatant IL-1β content ( C ) in control cells, Hp- 1 cells, Hp -30 cells, Hp -30 cells infected with H. pylori for 24 h and Hp -30 cells co-culture with H. pylori and 1 μg/ml Pam2CSK4 for 24 h. pJNK expression ( D ) in Hp- 30 cells, Hp- 30 cells co-culture with H. pylori for 24 h, Hp -30 cells co-culture with H. pylori and 1 μg/ml Pam2CSK4 for 24 h. All experiment were performed in triplicate and all data represents means ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The TLR2/6 heterodimer agonist Pam2CSK4 was purchased from Apexbio (B5665).

Techniques: Expressing, Co-Culture Assay, Control, Infection

Journal: Gastric Cancer

Article Title: Sustained exposure to Helicobacter pylori induces immune tolerance by desensitizing TLR6

doi: 10.1007/s10120-023-01461-7

Figure Lengend Snippet: Schematic ( A ) of the in vivo treatment experimental plan. Serum TLR6 and IL-8 expression ( B, C ) and gastric mucosa TLR6 and IL-8 IHC staining ( D , E ) of the H. pylori- negative Mongolian gerbils or in the H. pylori- infected gerbils after 24 h, 48 h and 72 h intraperitoneal administration of Pam2CSK4 or DMSO. pJNK expression ( F ) in the gastric mucosa of H. pylori- negative Mongolian gerbils or in the H. pylori- infected gerbils after 72 h intraperitoneal administration of Pam2CSK4 or DMSO. The Immunofluorescence staining of CD11b + /Ly6G + cells ( G ), monocyte/macrophage ( I ), the number of CD11b + /Ly6G + cells ( H ) and the number of monocyte/macrophage ( J ) in gastric mucosa of H. pylori -negative gerbils, H. pylori -positive gerbils treated with DMSO or Pam2CSK4. The level of H. pylori 23srRNA ( K ) in gastric mucosa of H. pylori -positive Mongolian gerbils treated with DMSO or Pam2CSK4. IHC staining of TLR6 expression ( L ) in the gastric mucosa of patients. × 100. The average optical density (AOD) of TLR6 expression ( M ) in patients’ gastric mucosa. All experiment were performed in triplicate and all data represents means ± SEM. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The TLR2/6 heterodimer agonist Pam2CSK4 was purchased from Apexbio (B5665).

Techniques: In Vivo, Expressing, Immunohistochemistry, Infection, Immunofluorescence, Staining